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Characterization of β-galactosidase in the Crude Plant Extract of Artemisia judaica L. in Presence and Absence of Some Heavy Metals

Received: 15 August 2016     Accepted: 25 August 2016     Published: 21 September 2016
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Abstract

β-galactosidase (EC 3.2.1.23) is important in the formation of a medicinal plant Artemisia judaica (al-ba’atharan) aroma. The crude plant extracts of Artemisia judaica were used to characterize the enzyme in the term of pH, temperature, enzyme kinetic and effects of some heavy metals on its activity. The enzyme activity was measured by its ability to hydrolyze the substrate 2-nitrophenyl β-D-galactopyranoside (ONPG). The enzyme activity was reached maximum at 50°C and at pH 6.0. The Km and Vmax values of the enzyme were 3.6 mM and 1.67 μmol/min, respectively. Uncompetitive inhibition was observed in presence of Hg+2, Fe+3 and Zn+2 for the enzyme β-galactosidase in the crude extract through the decrease in the Km and Vmax values. Pb+2 and Cu+2 were found to act as a noncompetitive inhibitors on the enzyme β-galactosidase in the crude extract due to increase in the Km values and decrease in Vmax values. The study showed that Hg+2 was the most potent inhibitor while Cu+2 exhibited the least inhibition degree on β-galactosidase activity in the Artemisia judaica. These finding indicated that the enzyme β-galactosidase in the crude leaves extract of Artemisia judaica can be used in industrial and medical applications.

Published in American Journal of Life Sciences (Volume 4, Issue 5)
DOI 10.11648/j.ajls.20160405.11
Page(s) 99-105
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Al-ba’atharan, β-galactosidase, Enzymatic Kinetics, Heavy Metals

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    Omar M. Atrooz, Mohammad H. Abukhalil, Ibrahim M. AlRawashdeh. (2016). Characterization of β-galactosidase in the Crude Plant Extract of Artemisia judaica L. in Presence and Absence of Some Heavy Metals. American Journal of Life Sciences, 4(5), 99-105. https://doi.org/10.11648/j.ajls.20160405.11

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    ACS Style

    Omar M. Atrooz; Mohammad H. Abukhalil; Ibrahim M. AlRawashdeh. Characterization of β-galactosidase in the Crude Plant Extract of Artemisia judaica L. in Presence and Absence of Some Heavy Metals. Am. J. Life Sci. 2016, 4(5), 99-105. doi: 10.11648/j.ajls.20160405.11

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    AMA Style

    Omar M. Atrooz, Mohammad H. Abukhalil, Ibrahim M. AlRawashdeh. Characterization of β-galactosidase in the Crude Plant Extract of Artemisia judaica L. in Presence and Absence of Some Heavy Metals. Am J Life Sci. 2016;4(5):99-105. doi: 10.11648/j.ajls.20160405.11

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  • @article{10.11648/j.ajls.20160405.11,
      author = {Omar M. Atrooz and Mohammad H. Abukhalil and Ibrahim M. AlRawashdeh},
      title = {Characterization of β-galactosidase in the Crude Plant Extract of Artemisia judaica L. in Presence and Absence of Some Heavy Metals},
      journal = {American Journal of Life Sciences},
      volume = {4},
      number = {5},
      pages = {99-105},
      doi = {10.11648/j.ajls.20160405.11},
      url = {https://doi.org/10.11648/j.ajls.20160405.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160405.11},
      abstract = {β-galactosidase (EC 3.2.1.23) is important in the formation of a medicinal plant Artemisia judaica (al-ba’atharan) aroma. The crude plant extracts of Artemisia judaica were used to characterize the enzyme in the term of pH, temperature, enzyme kinetic and effects of some heavy metals on its activity. The enzyme activity was measured by its ability to hydrolyze the substrate 2-nitrophenyl β-D-galactopyranoside (ONPG). The enzyme activity was reached maximum at 50°C and at pH 6.0. The Km and Vmax values of the enzyme were 3.6 mM and 1.67 μmol/min, respectively. Uncompetitive inhibition was observed in presence of Hg+2, Fe+3 and Zn+2 for the enzyme β-galactosidase in the crude extract through the decrease in the Km and Vmax values. Pb+2 and Cu+2 were found to act as a noncompetitive inhibitors on the enzyme β-galactosidase in the crude extract due to increase in the Km values and decrease in Vmax values. The study showed that Hg+2 was the most potent inhibitor while Cu+2 exhibited the least inhibition degree on β-galactosidase activity in the Artemisia judaica. These finding indicated that the enzyme β-galactosidase in the crude leaves extract of Artemisia judaica can be used in industrial and medical applications.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Characterization of β-galactosidase in the Crude Plant Extract of Artemisia judaica L. in Presence and Absence of Some Heavy Metals
    AU  - Omar M. Atrooz
    AU  - Mohammad H. Abukhalil
    AU  - Ibrahim M. AlRawashdeh
    Y1  - 2016/09/21
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajls.20160405.11
    DO  - 10.11648/j.ajls.20160405.11
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 99
    EP  - 105
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20160405.11
    AB  - β-galactosidase (EC 3.2.1.23) is important in the formation of a medicinal plant Artemisia judaica (al-ba’atharan) aroma. The crude plant extracts of Artemisia judaica were used to characterize the enzyme in the term of pH, temperature, enzyme kinetic and effects of some heavy metals on its activity. The enzyme activity was measured by its ability to hydrolyze the substrate 2-nitrophenyl β-D-galactopyranoside (ONPG). The enzyme activity was reached maximum at 50°C and at pH 6.0. The Km and Vmax values of the enzyme were 3.6 mM and 1.67 μmol/min, respectively. Uncompetitive inhibition was observed in presence of Hg+2, Fe+3 and Zn+2 for the enzyme β-galactosidase in the crude extract through the decrease in the Km and Vmax values. Pb+2 and Cu+2 were found to act as a noncompetitive inhibitors on the enzyme β-galactosidase in the crude extract due to increase in the Km values and decrease in Vmax values. The study showed that Hg+2 was the most potent inhibitor while Cu+2 exhibited the least inhibition degree on β-galactosidase activity in the Artemisia judaica. These finding indicated that the enzyme β-galactosidase in the crude leaves extract of Artemisia judaica can be used in industrial and medical applications.
    VL  - 4
    IS  - 5
    ER  - 

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Author Information
  • Department of Biological Sciences, Mutah University, Mutah, Jordan

  • Department of Biological Sciences, Mutah University, Mutah, Jordan

  • Biological Science Department, Al-Hussein Bin Talal University, Maa'n, Jordan

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