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Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids

Received: 12 June 2014     Accepted: 19 April 2015     Published: 6 May 2015
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Abstract

Poly unsaturated fatty acids (PUFAs) participate in activities of all organisms from energy source to structural components. Disruption of metabolism results in diseases like atherosclerosis and obesity. In this work, optimal conditions to production of arachidonic, eicosapentaenoic and gamma- linolenic acids has been conducted in the oil degrading fungi-Cephalosporium humicola IE, Mucor globosus 11 and Pythium irregulare LX isolated from soils in Absheron peninsula, Azerbaijan. The fermentation process carried out at physicochemical conditions, carbon and nitrogen sources, temperature, pH, rate of inoculum, enzyme activity, exogenous fat and phosphate; as well as the stressful situations. Growth in crude oil as optimal carbon source showed that suitable nitrogen was peptone for lipid biosynthesis. In stable temperature the optimal lipid productionoccurred at 28°C, whereas in “temperature shifting technique”, greater yields occurred at 28°C for 5 days followed by at 15°C for 2 days. C/N ratio ranged from 4 to 64 and the optimal medium for production was composed of 2.0% glucose and 0.25% yeast extract, with addition of 0.1% KH2PO4at neutral pH with 3% inoculum. Although the activities of the enzymes varied among the fungi, but the developmental profiles for all enzymes were practically similar. Additive fat and phosphate accelerated growth and enhanced PUFA production. Data showed that the procedure of adaptation of fungi motivated the increase of the rate of membrane phospholipids with a high quantity of PUFAs. This research is considered to be object of PUFA production as food supplements.

Published in American Journal of Life Sciences (Volume 3, Issue 1-3)

This article belongs to the Special Issue Industrial Biotechnology

DOI 10.11648/j.ajls.s.2015030103.12
Page(s) 5-15
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Ascomycetes, Zygomycetes, Oomycetes, Lipid, SCO, PUFA, Crude Oil, Omega

References
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    Mehdi Ghasemi, Yemen Atakishiyeva. (2015). Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids. American Journal of Life Sciences, 3(1-3), 5-15. https://doi.org/10.11648/j.ajls.s.2015030103.12

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    ACS Style

    Mehdi Ghasemi; Yemen Atakishiyeva. Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids. Am. J. Life Sci. 2015, 3(1-3), 5-15. doi: 10.11648/j.ajls.s.2015030103.12

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    AMA Style

    Mehdi Ghasemi, Yemen Atakishiyeva. Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids. Am J Life Sci. 2015;3(1-3):5-15. doi: 10.11648/j.ajls.s.2015030103.12

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  • @article{10.11648/j.ajls.s.2015030103.12,
      author = {Mehdi Ghasemi and Yemen Atakishiyeva},
      title = {Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids},
      journal = {American Journal of Life Sciences},
      volume = {3},
      number = {1-3},
      pages = {5-15},
      doi = {10.11648/j.ajls.s.2015030103.12},
      url = {https://doi.org/10.11648/j.ajls.s.2015030103.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2015030103.12},
      abstract = {Poly unsaturated fatty acids (PUFAs) participate in activities of all organisms from energy source to structural components. Disruption of metabolism results in diseases like atherosclerosis and obesity. In this work, optimal conditions to production of arachidonic, eicosapentaenoic and gamma- linolenic acids has been conducted in the oil degrading fungi-Cephalosporium humicola IE, Mucor globosus 11 and Pythium irregulare LX isolated from soils in Absheron peninsula, Azerbaijan. The fermentation process carried out at physicochemical conditions, carbon and nitrogen sources, temperature, pH, rate of inoculum, enzyme activity, exogenous fat and phosphate; as well as the stressful situations. Growth in crude oil as optimal carbon source showed that suitable nitrogen was peptone for lipid biosynthesis. In stable temperature the optimal lipid productionoccurred at 28°C, whereas in “temperature shifting technique”, greater yields occurred at 28°C for 5 days followed by at 15°C for 2 days. C/N ratio ranged from 4 to 64 and the optimal medium for production was composed of 2.0% glucose and 0.25% yeast extract, with addition of 0.1% KH2PO4at neutral pH with 3% inoculum. Although the activities of the enzymes varied among the fungi, but the developmental profiles for all enzymes were practically similar. Additive fat and phosphate accelerated growth and enhanced PUFA production. Data showed that the procedure of adaptation of fungi motivated the increase of the rate of membrane phospholipids with a high quantity of PUFAs. This research is considered to be object of PUFA production as food supplements.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Isolation, Identification and Optimization of Crude Oil Degrading Micromycetes for Biosynthesis of Poly Unsaturated Fatty Acids
    AU  - Mehdi Ghasemi
    AU  - Yemen Atakishiyeva
    Y1  - 2015/05/06
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ajls.s.2015030103.12
    DO  - 10.11648/j.ajls.s.2015030103.12
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
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    EP  - 15
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.s.2015030103.12
    AB  - Poly unsaturated fatty acids (PUFAs) participate in activities of all organisms from energy source to structural components. Disruption of metabolism results in diseases like atherosclerosis and obesity. In this work, optimal conditions to production of arachidonic, eicosapentaenoic and gamma- linolenic acids has been conducted in the oil degrading fungi-Cephalosporium humicola IE, Mucor globosus 11 and Pythium irregulare LX isolated from soils in Absheron peninsula, Azerbaijan. The fermentation process carried out at physicochemical conditions, carbon and nitrogen sources, temperature, pH, rate of inoculum, enzyme activity, exogenous fat and phosphate; as well as the stressful situations. Growth in crude oil as optimal carbon source showed that suitable nitrogen was peptone for lipid biosynthesis. In stable temperature the optimal lipid productionoccurred at 28°C, whereas in “temperature shifting technique”, greater yields occurred at 28°C for 5 days followed by at 15°C for 2 days. C/N ratio ranged from 4 to 64 and the optimal medium for production was composed of 2.0% glucose and 0.25% yeast extract, with addition of 0.1% KH2PO4at neutral pH with 3% inoculum. Although the activities of the enzymes varied among the fungi, but the developmental profiles for all enzymes were practically similar. Additive fat and phosphate accelerated growth and enhanced PUFA production. Data showed that the procedure of adaptation of fungi motivated the increase of the rate of membrane phospholipids with a high quantity of PUFAs. This research is considered to be object of PUFA production as food supplements.
    VL  - 3
    IS  - 1-3
    ER  - 

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Author Information
  • Institute of microbiology, Azerbaijan National Academy of Sciences, Baku, Azerbaijan

  • Institute of microbiology, Azerbaijan National Academy of Sciences, Baku, Azerbaijan

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