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In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants

Received: 29 April 2016     Accepted: 20 May 2016     Published: 17 June 2016
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Abstract

Dendrobium jerdonianum Wight is an Epiphytic tufted orchid at higher elevations. The maximum percentage establishment of Flower stalk, leaf, node and internode explants was observed on MS, VW and KC Medium. However, MS media fortified with activated charcoal exhibited maximum multiplication rate for in vitro rooting. MS, VW and KC media supplemented with various concentrations of auxins and cytokinins were used in flower stalk, leaf, nodes and internodes for plantlet formation. In the evaluation of the media MS basal medium fortified with 0.5mg 2, 4, D-dichloro phenoxy acetic acid +5mg BAP + 50mlsuitable for flower stalk culture. KC medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid was found to be suitable for Leaf and internodes Culture. VW medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 3mg BAP + 50ml CM, was found to be suitable for nodal segments. The entire above medium used with different composition gives highest percentage of 80-95 percent results for plantlet formation. In vitro rooting was successful with MS medium supplemented with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 5mg IAA + 50ml CM and 500mg of activated charcoal. 90 days old in vitro plantlets were hardened and transferred to green house after ex vitro rooting technique. Significance of the present work is discussed here.

Published in American Journal of Life Sciences (Volume 4, Issue 3)
DOI 10.11648/j.ajls.20160403.12
Page(s) 76-81
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Plantlet Formation, Explants, Dendrobium jerdonianum, Propagation, Establishment

References
[1] Ahmedullah, M. & Nayar M. P. (1987). Endemic Plants of the Indian region- Vol 1. Peninsular India, Botanical Survey of India, Calcutta, 262pp.
[2] Aktat, S. Nasiruddin, K. M. & Huq, H. (2007). In vitro root formation in Dendrobium orchid plantlets with IBA. J Agr. Rural Dev. 5: 48–51.
[3] Arditti, J. (1977). Clonal propagation of orchids by means of tissue culture-a manual. In J. Arditti (ed.). Orchid biology. vol. I, 203-293. Cornell University Press. New York. USA.
[4] Arditti, J. and Ernst, R. (1993). Micropropagation of Orchids. Wiley and Sons. New York. USA.
[5] Blatter, E. (1928). A list of orchids with some new species from High Wavy Mountain (Madurai District). Journal of the Bombay Natural History Society 32: 518–523
[6] CSS451 Plant Tissue Culture, (2010).
[7] Fay, M. E. (1994). In what situations is in vitro appropriate to plant conservation. Biodiv. Conserv. 3: 176–183.
[8] Govaerts et al., (2012). Endemic orchids of Western Ghats.
[9] Hooker, J. D. (1888–1890). Flora of British India. Vols. 5 & 6. L. Reeve & Co. London. 667–858 & 1–198pp.
[10] Jeewan Singh Jalal, & Jayanthi, J. (2012). Endemic orchids of peninsular India: a review, Botanical Survey of India, Western Regional Centre, Maharashtra 4 (15): 3415-3425.
[11] Mittermeier, (2000). Hotspots: Earth’s Biologically Richest and Most Endangered Terrestrial Eco regions. Cemex/Conservation International 431 pp.
[12] Mohanan, M. and Balakrishna, N. P. (1991). Endangered orchids of Nilgiris Biosphere Reserve India. Kerala Forest Department, Thiruvananthapuram. Pp. 187-199.
[13] Nayar, M. P. (1996). Hot Spots of Endemic Plants of India, Nepal and Bhutan. Tropical Botanic Garden and Research Institute. Thiruvananthapuram. 252pp.
[14] Orange Kerala Dendrobium, http://www.flowersofindia.net/)
[15] Park, SY., Murthy, H. N & Paek, K. Y (2002), Rapid propagation of Phalaenopsis from floral stalk-derived leaves. In vitro Cell Dev Biol Plant. 38 (2002) 168.
[16] Subramanian, K. & Nayar, M. P. (1974). Vegetation and phytogeography of the Western Ghats, pp. 178–196.
[17] W3 Tropicos. Kew Monocot list, International Plant Names Index (IPNI).
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  • APA Style

    Sr. Sagaya Mary B., Divakar K. M. (2016). In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants. American Journal of Life Sciences, 4(3), 76-81. https://doi.org/10.11648/j.ajls.20160403.12

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    ACS Style

    Sr. Sagaya Mary B.; Divakar K. M. In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants. Am. J. Life Sci. 2016, 4(3), 76-81. doi: 10.11648/j.ajls.20160403.12

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    AMA Style

    Sr. Sagaya Mary B., Divakar K. M. In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants. Am J Life Sci. 2016;4(3):76-81. doi: 10.11648/j.ajls.20160403.12

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  • @article{10.11648/j.ajls.20160403.12,
      author = {Sr. Sagaya Mary B. and Divakar K. M.},
      title = {In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants},
      journal = {American Journal of Life Sciences},
      volume = {4},
      number = {3},
      pages = {76-81},
      doi = {10.11648/j.ajls.20160403.12},
      url = {https://doi.org/10.11648/j.ajls.20160403.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160403.12},
      abstract = {Dendrobium jerdonianum Wight is an Epiphytic tufted orchid at higher elevations. The maximum percentage establishment of Flower stalk, leaf, node and internode explants was observed on MS, VW and KC Medium. However, MS media fortified with activated charcoal exhibited maximum multiplication rate for  in vitro rooting. MS, VW and KC media supplemented with various concentrations of auxins and cytokinins were used in flower stalk, leaf, nodes and internodes for plantlet formation. In the evaluation of the media MS basal medium fortified with 0.5mg 2, 4, D-dichloro phenoxy acetic acid +5mg BAP + 50mlsuitable for flower stalk culture. KC medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid was found to be suitable for Leaf and internodes Culture. VW medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 3mg BAP + 50ml CM, was found to be suitable for nodal segments. The entire above medium used with different composition gives highest percentage of 80-95 percent results for plantlet formation.  In vitro rooting was successful with MS medium supplemented with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 5mg IAA + 50ml CM and 500mg of activated charcoal. 90 days old  in vitro plantlets were hardened and transferred to green house after ex vitro rooting technique. Significance of the present work is discussed here.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - In vitro Propagation of Dendrobium jerdonianum Wight Through Flower Stalk, Leaf, Nodes/ Internodes as Explants
    AU  - Sr. Sagaya Mary B.
    AU  - Divakar K. M.
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    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
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    EP  - 81
    PB  - Science Publishing Group
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    UR  - https://doi.org/10.11648/j.ajls.20160403.12
    AB  - Dendrobium jerdonianum Wight is an Epiphytic tufted orchid at higher elevations. The maximum percentage establishment of Flower stalk, leaf, node and internode explants was observed on MS, VW and KC Medium. However, MS media fortified with activated charcoal exhibited maximum multiplication rate for  in vitro rooting. MS, VW and KC media supplemented with various concentrations of auxins and cytokinins were used in flower stalk, leaf, nodes and internodes for plantlet formation. In the evaluation of the media MS basal medium fortified with 0.5mg 2, 4, D-dichloro phenoxy acetic acid +5mg BAP + 50mlsuitable for flower stalk culture. KC medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid was found to be suitable for Leaf and internodes Culture. VW medium equipped with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 3mg BAP + 50ml CM, was found to be suitable for nodal segments. The entire above medium used with different composition gives highest percentage of 80-95 percent results for plantlet formation.  In vitro rooting was successful with MS medium supplemented with 0.5mg 2, 4, D-dichloro phenoxy acetic acid + 5mg IAA + 50ml CM and 500mg of activated charcoal. 90 days old  in vitro plantlets were hardened and transferred to green house after ex vitro rooting technique. Significance of the present work is discussed here.
    VL  - 4
    IS  - 3
    ER  - 

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Author Information
  • Plant Tissue Culture Division, Department of Botany, St. Joseph’s Post-Graduate Studies and Research Centre, Bangalore, India

  • Plant Tissue Culture Division, Department of Botany, St. Joseph’s Post-Graduate Studies and Research Centre, Bangalore, India

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