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Extraction of Some Secondary Metabolites &Thin Layer Chromatography from Different Parts of Centella Asiatica L. (URB)

Received: 30 August 2013     Published: 30 November 2013
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Abstract

Centella asiatica (L.) Urb. known as Brahmi, Indian Pennywort and Mandookaparni is a small herbaceous annual plant of the family Apiaceae, It is used in traditional medicine for the treatment of various ailments & ayurvedic treatment for many diseases. Present study deals with the qualitative and quantitative analysis of ethanolic extract of root, stem & leaf of Centella asiatica (L.) Urb. A qualitative analysis by thin layer chromatography & a quantitative analysis by standard chemical protocol of secondary metabolites in the root, stem and leaf of the Centella asiatica L. (URB) have been studied. Using thin layer chromatography (TLC) different components like Alkaloids, Saponin, Flavonoids, Terpenoides, Phenol & Tannin are isolated & identified. The Rf values of the developed spots in the different solvent systems are noted. In the quantitative analysis, alkaloids, saponins, terpenoids & flavonoids are extracted by using the standard chemical protocol. These results may be helpful for rationale use of this plant in the modern system of health care.

Published in American Journal of Life Sciences (Volume 1, Issue 6)
DOI 10.11648/j.ajls.20130106.11
Page(s) 243-247
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Centella Asiatica L. Quantitative Analysis, TLC

References
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[6] Veerapur VP, Prabhakar KR, Parihar VP, Kandadi MR, Ramakrishana S et al. Ficus racemosa Stem Bark Extract: A Potent Antioxidant and a Probable Natural Radioprotector. Evid Based Complement Alternat Med 2009; 6(3): 317-324.
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[8] Muselík J, García-Alonso M, Martín-López MP, Želmièka M, Rivas-Gonzalo JC. Measurement of Antioxidant Activity of Wine Catechins, Procyanidins, Antocyanins and Piranoantocyanins. Int J Mol Sci 2007; 8: 797-809.
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  • APA Style

    Sanjay R. Biradar, Bhagyashri D. Rachetti. (2013). Extraction of Some Secondary Metabolites &Thin Layer Chromatography from Different Parts of Centella Asiatica L. (URB). American Journal of Life Sciences, 1(6), 243-247. https://doi.org/10.11648/j.ajls.20130106.11

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    ACS Style

    Sanjay R. Biradar; Bhagyashri D. Rachetti. Extraction of Some Secondary Metabolites &Thin Layer Chromatography from Different Parts of Centella Asiatica L. (URB). Am. J. Life Sci. 2013, 1(6), 243-247. doi: 10.11648/j.ajls.20130106.11

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    AMA Style

    Sanjay R. Biradar, Bhagyashri D. Rachetti. Extraction of Some Secondary Metabolites &Thin Layer Chromatography from Different Parts of Centella Asiatica L. (URB). Am J Life Sci. 2013;1(6):243-247. doi: 10.11648/j.ajls.20130106.11

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  • @article{10.11648/j.ajls.20130106.11,
      author = {Sanjay R. Biradar and Bhagyashri D. Rachetti},
      title = {Extraction of Some Secondary Metabolites &Thin Layer Chromatography from Different Parts of Centella Asiatica L. (URB)},
      journal = {American Journal of Life Sciences},
      volume = {1},
      number = {6},
      pages = {243-247},
      doi = {10.11648/j.ajls.20130106.11},
      url = {https://doi.org/10.11648/j.ajls.20130106.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130106.11},
      abstract = {Centella asiatica (L.) Urb.  known as Brahmi, Indian Pennywort and Mandookaparni is a small herbaceous annual plant of the family Apiaceae, It is used in traditional medicine for the treatment of various ailments & ayurvedic treatment for many diseases. Present study deals with the qualitative and quantitative analysis of ethanolic extract of root, stem & leaf of Centella asiatica (L.) Urb.  A qualitative analysis by thin layer chromatography & a quantitative analysis by standard chemical protocol of secondary metabolites in the root, stem and leaf of the Centella asiatica L. (URB) have been studied. Using thin layer chromatography (TLC) different components like Alkaloids, Saponin, Flavonoids, Terpenoides, Phenol & Tannin are isolated & identified. The Rf values of the developed spots in the different solvent systems are noted. In the quantitative analysis, alkaloids, saponins, terpenoids & flavonoids are extracted by using the standard chemical protocol.  These results may be helpful for rationale use of this plant in the modern system of health care.},
     year = {2013}
    }
    

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    T1  - Extraction of Some Secondary Metabolites &Thin Layer Chromatography from Different Parts of Centella Asiatica L. (URB)
    AU  - Sanjay R. Biradar
    AU  - Bhagyashri D. Rachetti
    Y1  - 2013/11/30
    PY  - 2013
    N1  - https://doi.org/10.11648/j.ajls.20130106.11
    DO  - 10.11648/j.ajls.20130106.11
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 243
    EP  - 247
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20130106.11
    AB  - Centella asiatica (L.) Urb.  known as Brahmi, Indian Pennywort and Mandookaparni is a small herbaceous annual plant of the family Apiaceae, It is used in traditional medicine for the treatment of various ailments & ayurvedic treatment for many diseases. Present study deals with the qualitative and quantitative analysis of ethanolic extract of root, stem & leaf of Centella asiatica (L.) Urb.  A qualitative analysis by thin layer chromatography & a quantitative analysis by standard chemical protocol of secondary metabolites in the root, stem and leaf of the Centella asiatica L. (URB) have been studied. Using thin layer chromatography (TLC) different components like Alkaloids, Saponin, Flavonoids, Terpenoides, Phenol & Tannin are isolated & identified. The Rf values of the developed spots in the different solvent systems are noted. In the quantitative analysis, alkaloids, saponins, terpenoids & flavonoids are extracted by using the standard chemical protocol.  These results may be helpful for rationale use of this plant in the modern system of health care.
    VL  - 1
    IS  - 6
    ER  - 

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Author Information
  • Tissue Culture Laboratory, Department of Botany, Shri Chhatrapati Shivaji College Omerga – 413606, Dist. Osmanabad, (MS).INDIA

  • Tissue Culture Laboratory, Department of Botany, Shri Chhatrapati Shivaji College Omerga – 413606, Dist. Osmanabad, (MS).INDIA

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