| Peer-Reviewed

Hepatoprotective Activity of Ocimum americanum L Leaves against Paracetamol – Induced Liver Damage in Rats

Published: 10 April 2013
Views:       Downloads:
Abstract

This study was designed to investigate the hepatoprotective activity of aqueous extract of Ocimum ameri-canum leaves against paracetamol – induced liver damage in rats. Hepatic damage was induced by paracetamol. Thereafter, the levels of some serum biochemical parameters such as alanine trasaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), albumin, total bilirubin (TBIL) and total protein (TP) were investigated. The activities of ALP, AST, ALT and histological changes in the liver of rats were also determined. Silymarin was used as the standard hepatoprotective drug. The pre – treatment of rats with aqueous extract of O. americanum leaves caused a significant increase in the serum levels of TP and albumin. There was a significant decrease in the serum levels of ALP, AST, ALT and TBIL with a corresponding increase in the activities of ALP, AST and ALT in the liver of extract treated rats. The hepatoprotection was confirmed by histological examinations of liver sections of normal and treated rats. Furthermore, rats intoxicated with paracetamol alone had their serum ALP, AST, ALT and TBIL levels significantly increased, while TP and albumin concentrations decreased when compared with the normal rats. The aqueous extract of Ocimum americanum leaves at doses of 200 and 400 mg /kg p.o. have significant hepatoprotective ability against paracetamol – induced hepatic damage in rats.

Published in American Journal of Life Sciences (Volume 1, Issue 2)
DOI 10.11648/j.ajls.20130102.13
Page(s) 37-42
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Ocimum americanum, Hepatoprotective Activity, Paracetamol, Biochemical Parameters

References
[1] Ibrahim M, Khaja MN and Aara A. Hepatoprotective activity of Sapindus mukorossi and Rheum emodi extracts: in vitro and in vivo studies. World J. Gastroenterology. 2008; 14 (16): 2566–2571.
[2] Arhoghro EM, Ekpo KE, Anosike EO, and Ibeh GO. Ef-fect of aqueous extract of bitter leaf (Vernonia Amygdalina Del) on carbon tetrachloride (CCl4) induced liver damage in albino wistar rats, European J. Scientific Res. 2009; 26 (1): 122–130.
[3] Pramyothin P, Ngamtin C, Poungshompoo S, and Chai-chantipyuth C. Hepatoprotective activity of Phyllanthus amarus Schum. et. Thonn. extract in ethanol treated rats: in vitro and in vivo studies," J. Ethnopharmacol. 2007; 114, (2): 169–173.
[4] Shah VN and Deval K. Hepatoprotective activity of leaves of Parkinsonia aculeata linn against paracetamol induced hepatotoxicity in rats. Int J Pharma 2011; 1(2): 59-66.
[5] Steel J." Perfumeros and the sacred use of fragrance in ama-zonian shamanism". The smell culture reader, edited by Jim Drobnick Berg publishers. 2006; Pp 23.
[6] Hogarh, NJ. Effect of Ocimum canum aqueous extract on experimental diabetes mellitus. BSc Research Project Report, Department of Biochemistry, University of Ghana, 1996.
[7] Bassole IHN, Nebie R, Savadogo A, Ouattara CT, Barro N and Traore SA. Composition and antimicrobial activities of the leaf and flower essential oils of Lippia chevalieri and Ocimum canum from Bukina faso. Afr. J. Biotech. 2005; 4 (10): 1156-1160
[8] Oboh, G. Antioxidative potential of Ocimum gratissimum and Ocimum canum leaf polyphenols and protective effects on some pro-oxidants induced lipid peroxidation in rat brain: An in vitro study. American J. Food Technol. 2008; 3 (5): 325-334.
[9] OECD, "Test 423: acute oral toxicity—acute toxic class method," OECD Guidelines for the Testing of Chemicals, 2011; 1 (4): 1–14.
[10] Nirmala M, Girija K, Lakshman K and Divya T. Hepatopro-tective activity of Musa paradisiaca on experimental animal models. Asian Pacific J. Tropic. Biomed. 2012; 11-15.
[11] Shannon MK, Gallardo-Romero NF, Gregory LL, Damon IK, Kevin LK and Carroll DS. Physiologic Reference Ranges for Captive Black- Tailed Prairie Dogs (Cynomys ludovicianus). Journal of the American Association for La-boratory Animal Science. 2010; 49 (3): 274 – 281.
[12] Opoku AR, Ndlovu IM, Terblanche SE, and Hutchings AH, "In vivo hepatoprotective effects of Rhoicissus tridentata subsp. cuneifolia, a traditional Zulumedicinal plant against CCl4-induced acute liver injury in rats. South African J. Bo-tany. 2007; 73 (3): 372–377.
[13] Zakaria ZA, Rofiee MS,. Somchit MN, Zuraini A, Sulaiman MR, The LK, Salleh MZ and Long K. Hepatoprotective Ac-tivity of Dried- and Fermented-Processed Virgin Coconut Oil. Evid. Based Complement. Altern. Med. 2011; 1 – 8.
[14] Somchit MN, Zuraini A, Ahmad Bustaman A, Somchit N, Sulaiman MR, and Noratunlina R. Protective activity of turmeric (Curcuma longa) in paracetamol-induced hepato-toxicity in rats, Inter. J. Pharmacol. 2005; 1 (3): 252–256.
[15] Hazai E, Vereczkey L, and Monostory K. Reduction of toxic metabolite formation of acetaminophen," Biochem. Biophy. Res. Comm. 2002 291 (4): 1089–1094.
[16] Dash DK, Yeligar VC, and Nayak SS. Evaluation of hepato-protective and antioxidant activity of Ichnocarpus frutescens (Linn.) R.Br. on paracetamol-induced hepatotoxicity in rats. Tropical J. Pharmaceu. Res. 2007; 6 (3): 755–765.
[17] Manokaran S, Jaswanth A, Sengottuvelu S, Nandhakumar J, Duraisamy R and Karthikeyan D. Hepatoprotective activity of Aerva lanata Linn. against paracetamol induced hepatotoxicity in rats. Res J Pharm Tech 2008; 1(4): 398-400.
[18] Tiwari BK and Khosa RL. Hepatoprotective and antioxidant effect of Sphaeranthus indicus against acetaminophen-induced hepatotoxicity in rats. J Pharm Sci Res 2009; 1(2): 26-30.
[19] Pendota SC, Yakubu MT, Grierson DS and Afolayan AJ. Effect of administration of aqueous extract of Hippobromus pauciflorus leaves in male wistar rats. Afr. J. Trad. CAM 2010; 7 (1): 40 – 46.
[20] Afolayan AJ and Yakubu MT. Effect of bulbine natalensis baker stem extract on the functional indices and histology of the liver and kidney of male wistar rats J Med Food 2009; 12 (4): 814–820
[21] Sasidharan S, Aravindran S, Latha LY, Vijenthi R, Saravanan D, and Amutha S, In vitro antioxidant activity and hepa-toprotective effects of lentinula edodes against paracetamol induced hepatotoxicity, Molecules 2010; 15 (6): 4478– 4489.
[22] Kumar G, Banu GS, Pappa PV, Sundararajan M, and Pan-dian MR, "Hepatoprotective activity of Trianthema portula-castrum L. against paracetamol and thioacetamide intoxication in albino rats," J. Ethnopharmacol. 2004; 92 (1): 37–40.
[23] Saha P, Mazumder UK, Haldar PK, Bala A, Kar B and Naskar S. Evaluation of Hepatoprotective activity of Cucur-bita maxima aerial parts. J Herbal Med Toxicol 2011; 5: 17-22.
Cite This Article
  • APA Style

    B. T. Aluko, O. I. Oloyede, A. J. Afolayan. (2013). Hepatoprotective Activity of Ocimum americanum L Leaves against Paracetamol – Induced Liver Damage in Rats. American Journal of Life Sciences, 1(2), 37-42. https://doi.org/10.11648/j.ajls.20130102.13

    Copy | Download

    ACS Style

    B. T. Aluko; O. I. Oloyede; A. J. Afolayan. Hepatoprotective Activity of Ocimum americanum L Leaves against Paracetamol – Induced Liver Damage in Rats. Am. J. Life Sci. 2013, 1(2), 37-42. doi: 10.11648/j.ajls.20130102.13

    Copy | Download

    AMA Style

    B. T. Aluko, O. I. Oloyede, A. J. Afolayan. Hepatoprotective Activity of Ocimum americanum L Leaves against Paracetamol – Induced Liver Damage in Rats. Am J Life Sci. 2013;1(2):37-42. doi: 10.11648/j.ajls.20130102.13

    Copy | Download

  • @article{10.11648/j.ajls.20130102.13,
      author = {B. T. Aluko and O. I. Oloyede and A. J. Afolayan},
      title = {Hepatoprotective Activity of Ocimum americanum L Leaves against Paracetamol – Induced Liver Damage in Rats},
      journal = {American Journal of Life Sciences},
      volume = {1},
      number = {2},
      pages = {37-42},
      doi = {10.11648/j.ajls.20130102.13},
      url = {https://doi.org/10.11648/j.ajls.20130102.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20130102.13},
      abstract = {This study was designed to investigate the hepatoprotective activity of aqueous extract of Ocimum ameri-canum leaves against paracetamol – induced liver damage in rats. Hepatic damage was induced by paracetamol. Thereafter, the levels of some serum biochemical parameters such as alanine trasaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), albumin, total bilirubin (TBIL) and total protein (TP) were investigated. The activities of ALP, AST, ALT and histological changes in the liver of rats were also determined. Silymarin was used as the standard hepatoprotective drug. The pre – treatment of rats with aqueous extract of O. americanum leaves caused a significant increase in the serum levels of TP and albumin. There was a significant decrease in the serum levels of ALP, AST, ALT and TBIL with a corresponding increase in the activities of ALP, AST and ALT in the liver of extract treated rats. The hepatoprotection was confirmed by histological examinations of liver sections of normal and treated rats. Furthermore, rats intoxicated with paracetamol alone had their serum ALP, AST, ALT and TBIL levels significantly increased, while TP and albumin concentrations decreased when compared with the normal rats.  The aqueous extract of Ocimum americanum leaves at doses of 200 and 400 mg /kg p.o. have significant hepatoprotective ability against paracetamol – induced hepatic damage in rats.},
     year = {2013}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Hepatoprotective Activity of Ocimum americanum L Leaves against Paracetamol – Induced Liver Damage in Rats
    AU  - B. T. Aluko
    AU  - O. I. Oloyede
    AU  - A. J. Afolayan
    Y1  - 2013/04/10
    PY  - 2013
    N1  - https://doi.org/10.11648/j.ajls.20130102.13
    DO  - 10.11648/j.ajls.20130102.13
    T2  - American Journal of Life Sciences
    JF  - American Journal of Life Sciences
    JO  - American Journal of Life Sciences
    SP  - 37
    EP  - 42
    PB  - Science Publishing Group
    SN  - 2328-5737
    UR  - https://doi.org/10.11648/j.ajls.20130102.13
    AB  - This study was designed to investigate the hepatoprotective activity of aqueous extract of Ocimum ameri-canum leaves against paracetamol – induced liver damage in rats. Hepatic damage was induced by paracetamol. Thereafter, the levels of some serum biochemical parameters such as alanine trasaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), albumin, total bilirubin (TBIL) and total protein (TP) were investigated. The activities of ALP, AST, ALT and histological changes in the liver of rats were also determined. Silymarin was used as the standard hepatoprotective drug. The pre – treatment of rats with aqueous extract of O. americanum leaves caused a significant increase in the serum levels of TP and albumin. There was a significant decrease in the serum levels of ALP, AST, ALT and TBIL with a corresponding increase in the activities of ALP, AST and ALT in the liver of extract treated rats. The hepatoprotection was confirmed by histological examinations of liver sections of normal and treated rats. Furthermore, rats intoxicated with paracetamol alone had their serum ALP, AST, ALT and TBIL levels significantly increased, while TP and albumin concentrations decreased when compared with the normal rats.  The aqueous extract of Ocimum americanum leaves at doses of 200 and 400 mg /kg p.o. have significant hepatoprotective ability against paracetamol – induced hepatic damage in rats.
    VL  - 1
    IS  - 2
    ER  - 

    Copy | Download

Author Information
  • Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria

  • Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria

  • Department of Botany, University of Fort Hare, Alice, South Africa

  • Sections